Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation

Abstract Background During the process of deep decay, when decay approaches pulp, an immune response is triggered inside which activates complement cascade. The effect component 5a (C5a) on differentiation dental pulp mesenchymal stem cells (DPSCs) related to dentin reparation. aim present study was stimulate DPSCs with different concentrations C5a and evaluate odontoblasts using sialoprotein (DSP). Methods were divided into following six groups: (i) Control; (ii) treated 50 ng/ml C5a; (iii) 100 (iv) 200 (v) 300 (vi) 400 C5a. Flow cytometry multilineage potential used identify DPSCs. Mineralization induction, Real-time PCR Western blot conducted odontoblast in 6 groups. Result can express cell markers, including CD105, CD90, CD73 and, a less common marker, stromal antigen-1. In addition, differentiate adipocytes, neurocytes, chondrocytes odontoblasts. All groups formed mineralized nodules after 28 days culture. Reverse transcription-quantitative western blotting indicated that high concentration expressed higher DSP levels promoted DPSC differentiation, whereas low displayed inhibitory effect. Conclusion this study, increasing C5a, accompanies has demonstrated enhancing at vitro.